Milton M. Sholley, Ph.D.
Office Phone: 804-828-9554
- B.S., Natural Sciences, Muhlenberg College (1969)
- Ph.D., Anatomy, Temple University School of Medicine (1974)
- Postdoctoral training in experimental pathology at Harvard University
Research and Scholarly Interests
In my laboratory we study the pathophysiology of the endothelial cells that line blood vessels. We use the human umbilical vein endothelial cell (HUVEC) culture system to model endothelial injuries that simulate those that occur during inflammation associated with ischemia/reperfusion injury and sepsis in vivo. Our investigations during the past few years have involved cellular and molecular mechanisms that alter the endothelium during cytokine-induced activation and oxidative injury. Our studies of oxidative injury have addressed the role of 72 kDa heat shock proteins in protecting endothelial cells from oxidative stress during exposure to hydrogen peroxide and the contributions of necrotic and apoptotic mechanisms of cell death . We also have studied modified expression of endothelial cellular adhesion molecules (CAMS) after activation with inflammatory cytokines. Recently, we have begun studies of the possible role of homeobox (HOX) gene products as upstream mediators of CAM expression in cytokine-activated HUVEC.
I am also active in the development of computer-assisted instruction programs used for the training of medical and graduate students in Human Gross Anatomy.
- Sholley, M.M., G.P. Ferguson, H.R. Seibel, J.L. Montour, and J.D. Wilson. Mechanisms of neovascularization: Vascular sprouting can occur without proliferation of endothelial cells. Lab. Invest. 51:624-634, 1984.
- Sholley, M.M. VIEWPOINT Small Group Preclinical Instruction: Methods within the Traditional Gross Anatomy Laboratory. Clinical Anatomy 7: 370-372, 1994.
- Gill, R.R. C.J. Gbur, Jr., B.J. Fisher, M.L. Hess, A.A. Fowler, III, R.C. Kukreja, and M.M. Sholley. Heat Shock Provides Delayed Protection against Oxidative Injury in Cultured Human Umbilical Vein Endothelial Cells. J. Mol. Cell. Card. 30:2739-2749, 1998.